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Elisa Test Kit ya Ochratoxin Chithunzi Chowonetsedwa
  • Elisa Test Kit ya Ochratoxin A
  • Elisa Test Kit ya Ochratoxin A

Elisa Test Kit ya Ochratoxin A

Kufotokozera Kwachidule:

Zidazi zitha kugwiritsidwa ntchito posanthula kuchuluka komanso kukwanira kwa ochratoxin A muzakudya.Ndi chinthu chatsopano chozindikira zotsalira za mankhwala pogwiritsa ntchito ukadaulo wa ELISA, womwe umangotengera 30min pa opareshoni iliyonse ndipo ukhoza kuchepetsa kwambiri zolakwika zogwirira ntchito komanso kuchuluka kwa ntchito.Chida ichi chimachokera paukadaulo wampikisano wa ELISA.Zitsime za microtiter zimakutidwa ndi antigen yolumikizana.Ochratoxin A mu zitsanzo amapikisana ndi antigen yokutidwa pa mbale ya microtiter ya antibody yomwe yawonjezeredwa.Pambuyo pakuwonjezera kwa enzyme conjugate, gawo lapansi la TMB limagwiritsidwa ntchito kuwonetsa mtundu.Kutaya kwachitsanzo kumagwirizana molakwika ndi o chratoxin A zotsalira m'menemo, pambuyo poyerekezera ndi Standard Curve, kuchulukitsidwa ndi zinthu zowonongeka, kuchuluka kwa Ochratoxin A mu chitsanzo kungawerengedwe.


Malangizo Ogwiritsa Ntchito

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Ochratoxins ndi gulu la mycotoxins opangidwa ndi mitundu ina ya Aspergillus (makamaka A).Ochratoxin A amadziwika kuti amapezeka muzinthu monga chimanga, khofi, zipatso zouma ndi vinyo wofiira.Imatengedwa ngati carcinogen yamunthu ndipo ili ndi chidwi chapadera chifukwa imatha kudziunjikira mu nyama yanyama.Choncho nyama ndi nyama zikhoza kuipitsidwa ndi poizoniyu.Kuwonetsedwa ndi ma ochratoxins kudzera muzakudya kumatha kukhala ndi chiwopsezo chambiri ku impso zoyamwitsa, ndipo kumatha kuyambitsa khansa.

Tsatanetsatane

1. Elisa Test Kit ya Ochratoxin A

2. Mphaka.Zithunzi za KA07301H-96

3. Zigawo za Kit
● mbale ya Microtiter yokhala ndi zitsime 96 zokutidwa ndi antigen
● Njira zokhazikika (mabotolo 6: 1ml/botolo)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
● Enzyme conjugate 7ml……………………………………………………………………..………..
● Antibody solution 10ml…………………………………………………………………………….…kapu yobiriwira
● Substrate solution A 7ml…………………………………………………………………………………kapu yoyera
● Substrate Solution B 7ml………………………………………………………………..…………………kapu yofiira
● Stop solution 7ml ………………………………………………………………………………………kapu yachikasu
● 20×concentrated Wash solution 40ml………..…………………………………….………kapu yowonekera

4. Kumverera, kulondola ndi kulondola
Kuzindikira Kuyesa: 0.4ppb
Malire ozindikira
Feed…………………………………………………………………………………………….… 5ppb
Kulondola
Chakudya ………………………………………………………………………….….….… 90±20%
Kulondola:Kusiyanasiyana kwa zida za ELISA ndizochepera 10%.

5. Cross Rate
Ochratoxin A………………………………………………………………………..100%

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