mankhwala

Mphaka.Chithunzi cha KB01009K-50T

Za
Izi zida ntchito mofulumira Mkhalidwe kusanthula tetracyclines uchi chitsanzo.

Njira yokonzekera chitsanzo
(1) Ngati uchi chitsanzo crystallized, kutenthetsa mu osamba madzi osapitirira 60 ℃, mpaka uchi chitsanzo thaw, kusakaniza kwathunthu, kuzirala monga firiji, ndiye kulemera kwa assay.
(2) Yesani 1.0 ± 0.05g homogenate mu chubu cha 10ml polystyrene centrifuge, onjezerani 3ml chitsanzo chotsitsa njira, vortex kwa 2min kapena gwedezani ndi dzanja mpaka chitsanzocho chisakanizidwe kwathunthu.

Zoyeserera.
(1.) Tengani mabotolo ofunikira mu phukusi la zida, chotsani makadi ofunikira, ndipo lembani zizindikiro zoyenera.Chonde gwiritsani ntchito makhadi oyeserera pasanathe 1h mukatsegula phukusi.
(2.) Tengani 100l okonzeka chitsanzo mu dzenje chitsanzo ndi pipette, ndiye yambani nthawi pambuyo madzi otaya.
(3.) Yalirani kwa 10min pa kutentha kwa firiji.
7.LOD

Zotsatira
Pali mizere iwiri m'dera la zotsatira za khadi, Control line ndi Tetracylcines Line, yomwe ili mwachidule monga "B" ndi "T".Zotsatira za mayeso zidzadalira mtundu wa mizere iyi.Chithunzi chotsatirachi chikufotokoza chizindikiritso cha zotsatira.
Zoyipa: Mzere wowongolera ndi mzere Woyesera zonse ndi zofiira ndipo T Line ndi mdima kuposa mzere wowongolera;
Tetracyclines Positive: Control Line ndi yofiira, T Line ilibe mtundu kapena T Line ndi yopepuka kuposa C line, kapena T Line ndi yofanana ndi C Line.

Kusungirako
2-30 ° C m'malo amdima owuma, musawume.Zidazi zitha kugwira ntchito pakadutsa miyezi 12.Nambala ya maere ndi tsiku lotha ntchito zimasindikizidwa pa phukusi.